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Editor: damberger
Time: 2014/11/08 00:43:23 GMT+1 |
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Note: |
changed: - Your WriteAssignment.lua dialog should look something like this <img src="06-ProteinB-WriteAssignmentsSettings.png"/><BR> Your WriteAssignment.lua dialog should look something like this StructureCalculationsOfComplexesWriteAssignmentSettings
Determining the structures of complexes or oligomers requires some additional procedures. Typically isotope-labeling of individual components helps to simplify the problem. The project is therefore divided into subprojects where only the resonance assignments of the isotope-labeled component are determined. Later the assignments of the individual components must be merged so that the NOEs between components can be analysed using experiments which use isotope-filtering and -editing to obtain only intermolecular NOEs.
CARA provides support for this analysis by allowing the definition of samples with different isotope-labeling patterns Moreover, SpinLinks? which represent NOEs? between spins can be defined to only appear in a subset of NOESY spectra. Via the SpinLink? visibility the subset of NOEs? visible in isotope-filtered/edited experiments can be represented. (See release notes for how to do this, further information is forthcoming on the wiki).
number by ChainId
c. Enter a number in the "Add offset to SpinId?" field that is larger than the maximum SpinId? determined in step 4 (e.g. 5000)
d. Select format CYANA.
e. Click OK (this writes out a sequence file starting at the ChainNr? of the first residue of protein B, and a chemical shift list .prot where each spin has an Id in the output file which is +5000 compared to the Id it has in Project B. We do this so that the sequence and chemical shift list can be merged with that of project A.
Your WriteAssignment?.lua dialog should look something like this StructureCalculationsOfComplexesWriteAssignmentSettings?
6. Duplicate project ProteinA (renaming it something like ProteinAandB).